4otu

Crystal structure of the gamma-glutamyltranspeptidase from Bacillus licheniformis in complex with L-Glutamate

spinqualitylabelsall models 4otu, resolution 3.02Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4otu is a 2 chain structure with sequence from Bacillus licheniformis and Bacillus licheniformis DSM 13 = ATCC 14580. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.022Å Ligands: GLU, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A9YTT0_BACLI Publication Abstract from PubMed gamma-Glutamyltranspeptidases (gamma-GTs) cleave the gamma-glutamyl amide bond of glutathione and transfer the released gamma-glutamyl group to water (hydrolysis) or acceptor amino acids (transpeptidation). These ubiquitous enzymes play a key role in the biosynthesis and degradation of glutathione, and in xenobiotic detoxification. Here we report the 3A resolution crystal structure of Bacillus licheniformis gamma-GT (BlGT) and that of its complex with l-Glu. X-ray structures confirm that BlGT belongs to the N-terminal nucleophilic hydrolase superfamily and reveal that the protein possesses an opened active site cleft similar to that reported for the homologous enzyme from Bacillus subtilis, but different from those observed for human gamma-GT and for gamma-GTs from other microorganisms. Data suggest that the binding of l-Glu induces a reordering of the C-terminal tail of BlGT large subunit and allow the identification of a cluster of acid residues that are potentially involved in the recognition of a metal ion. The role of these residues on the conformational stability of BlGT has been studied by characterizing the autoprocessing, enzymatic activity, chemical and thermal denaturation of four new Ala single mutants. The results show that replacement of Asp568 with an Ala affects both the autoprocessing and structural stability of the protein. Low resolution X-ray structure of gamma-glutamyltranspeptidase from Bacillus licheniformis: Opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion.,Lin LL, Chen YY, Chi MC, Merlino A Biochim Biophys Acta. 2014 Apr 26;1844(9):1523-1529. doi:, 10.1016/j.bbapap.2014.04.016. PMID:24780583 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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