4p76

Cellular response to a crystal-forming protein

spinqualitylabelsall models 4p76, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4p76 is a 2 chain structure with sequence from Dipsastraea favus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.9Å Ligands: CR8, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q53UG8_DIPFA Publication Abstract from PubMed Crystallization of proteins may occur in the cytosol of a living cell, but how a cell responds to intracellular protein crystallization remains unknown. We developed a variant of coral fluorescent protein that forms diffraction-quality crystals within mammalian cells. This expression system allowed the direct determination of its crystal structure at 2.9 A, as well as observation of the crystallization process and cellular responses. The micron-sized crystal, which emerged rapidly, was a pure assembly of properly folded beta-barrels and was recognized as an autophagic cargo that was transferred to lysosomes via a process involving p62 and LC3. Several lines of evidence indicated that autophagy was not required for crystal nucleation or growth. These findings demonstrate that in vivo protein crystals can provide an experimental model to study chemical catalysis. This knowledge may be beneficial for structural biology studies on normal and disease-related protein aggregation. A diffraction-quality protein crystal processed as an autophagic cargo.,Tsutsui H, Jinno Y, Shoda K, Tomita A, Matsuda M, Yamashita E, Katayama H, Nakagawa A, Miyawaki A Mol Cell. 2015 Apr 2;58(1):186-93. doi: 10.1016/j.molcel.2015.02.007. Epub 2015, Mar 12. PMID:25773597 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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