4qju
Crystal structure of DNA-bound nucleoid associated protein, SAV1473
Structural highlights
FunctionDBH_STAAM Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Publication Abstract from PubMedHU, one of the major nucleoid-associated proteins, interacts with the minor groove of DNA in a nonspecific manner to induce DNA bending or to stabilize bent DNA. In this study, crystal structures are reported for both free HU from Staphylococcus aureus Mu50 (SHU) and SHU bound to 21-mer dsDNA. The structures, in combination with electrophoretic mobility shift assays (EMSAs), isothermal titration calorimetry (ITC) measurements and molecular-dynamics (MD) simulations, elucidate the overall and residue-specific changes in SHU upon recognizing and binding to DNA. Firstly, structural comparison showed the flexible nature of the beta-sheets of the DNA-binding domain and that the beta-arms bend inwards upon complex formation, whereas the other portions are nearly unaltered. Secondly, it was found that the disruption and formation of salt bridges accompanies DNA binding. Thirdly, residue-specific free-energy analyses using the MM-PBSA method with MD simulation data suggested that the successive basic residues in the beta-arms play a central role in recognizing and binding to DNA, which was confirmed by the EMSA and ITC analyses. Moreover, residue Arg55 resides in the hinge region of the flexible beta-arms, exhibiting a remarkable role in their flexible nature. Fourthly, EMSAs with various DNAs revealed that SHU prefers deformable DNA. Taken together, these data suggest residue-specific roles in local shape and base readouts, which are primarily mediated by the flexible beta-arms consisting of residues 50-80. beta-Arm flexibility of HU from Staphylococcus aureus dictates the DNA-binding and recognition mechanism.,Kim do H, Im H, Jee JG, Jang SB, Yoon HJ, Kwon AR, Kang SM, Lee BJ Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3273-89. doi:, 10.1107/S1399004714023931. Epub 2014 Nov 28. PMID:25478845[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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