4r73

Structure of the periplasmic binding protein AfuA from Actinobacillus pleuropneumoniae (endogenous glucose-6-phosphate and mannose-6-phosphate bound)

spinqualitylabelsall models 4r73, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4r73 is a 2 chain structure with sequence from Actinobacillus pleuropneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: CL, G6P, GOL, M6P Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A3N294_ACTP2 Publication Abstract from PubMed Efficient acquisition of extracellular nutrients is essential for bacterial pathogenesis, however the identities and mechanisms for transport of many of these substrates remain unclear. Here, we investigate the predicted iron-binding transporter AfuABC and its role in bacterial pathogenesis in vivo. By crystallographic, biophysical and in vivo approaches, we show that AfuABC is in fact a cyclic hexose/heptose-phosphate transporter with high selectivity and specificity for a set of ubiquitous metabolites (glucose-6-phosphate, fructose-6-phosphate and sedoheptulose-7-phosphate). AfuABC is conserved across a wide range of bacterial genera, including the enteric pathogens EHEC O157:H7 and its murine-specific relative Citrobacter rodentium, where it lies adjacent to genes implicated in sugar sensing and acquisition. C. rodentium DeltaafuA was significantly impaired in an in vivo murine competitive assay as well as its ability to transmit infection from an afflicted to a naive murine host. Sugar-phosphates were present in normal and infected intestinal mucus and stool samples, indicating that these metabolites are available within the intestinal lumen for enteric bacteria to import during infection. Our study shows that AfuABC-dependent uptake of sugar-phosphates plays a critical role during enteric bacterial infection and uncovers previously unrecognized roles for these metabolites as important contributors to successful pathogenesis. Active Transport of Phosphorylated Carbohydrates Promotes Intestinal Colonization and Transmission of a Bacterial Pathogen.,Sit B, Crowley SM, Bhullar K, Lai CC, Tang C, Hooda Y, Calmettes C, Khambati H, Ma C, Brumell JH, Schryvers AB, Vallance BA, Moraes TF PLoS Pathog. 2015 Aug 21;11(8):e1005107. doi: 10.1371/journal.ppat.1005107., eCollection 2015 Aug. PMID:26295949 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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