4rdy

Crystal structure of VmoLac bound to 3-oxo-C10 AHL

spinqualitylabelsall models 4rdy, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rdy is a 2 chain structure with sequence from Vulcanisaeta moutnovskia 768-28. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: 3M5, CO, GOL, KCX, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F0QXN6_VULM7 Publication Abstract from PubMed A new representative of the Phosphotriesterase-Like Lactonases (PLLs) family from the hyperthermophilic crenarchaeon Vulcanisaeta moutnovskia has been characterized and crystallized. VmoLac is a native, proficient lactonase with promiscuous, low phosphotriesterase activity. VmoLac therefore represents an interesting candidate for engineering studies, with the aim of developing an efficient bacterial quorum-quenching agent. Here, we provide an extensive biochemical and kinetic characterization of VmoLac and describe the X-ray structures of the enzyme bound to a fatty acid and to its cognate substrate 3-oxo-C10 AHL (Acyl-Homoserine Lactone). The structures highlight possible structural determinants that may be involved in its extreme thermal stability (Tm = 128 degrees C). Moreover, the structure reveals that the substrate binding mode of VmoLac significantly differs from those of its close homologues, possibly explaining the substrate specificity of the enzyme. Finally, we describe the specific interactions between the enzyme and its substrate, and discuss the possible lactone hydrolysis mechanism of VmoLac. Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia.,Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M Sci Rep. 2015 Feb 11;5:8372. doi: 10.1038/srep08372. PMID:25670483 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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