4rg4

Epsilon-caprolactone-bound crystal structure of cyclohexanone monooxygenase in the Loose conformation

spinqualitylabelsall models 4rg4, resolution 2.51Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rg4 is a 1 chain structure with sequence from Rhodococcus sp. HI-31. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.51Å Ligands: ECE, FAD, NAP, PTD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C0STX7_9NOCA Publication Abstract from PubMed The Baeyer-Villiger monooxygenases (BVMOs) are microbial enzymes that catalyze the synthetically useful Baeyer-Villiger oxidation reaction. The available BVMO crystal structures all lack a substrate or product bound in a position that would determine the substrate specificity and stereospecificity of the enzyme. Here, we report two crystal structures of cyclohexanone monooxygenase (CHMO) with its product, epsilon-caprolactone, bound: the CHMOTight and CHMOLoose structures. The CHMOTight structure represents the enzyme state in which substrate acceptance and stereospecificity is determined, providing a foundation for engineering BVMOs with altered substrate spectra and/or stereospecificity. The CHMOLoose structure is the first structure where the product is solvent accessible. This structure represents the enzyme state upon binding and release of the substrate and product. In addition, the role of the invariant Arg329 in chaperoning the substrate/product during the catalytic cycle is highlighted. Overall, these data provide a structural framework for the engineering of BVMOs with altered substrate spectra and/or stereospecificity. Lactone-bound structures of cyclohexanone monooxygenase provide insight into the stereochemistry of catalysis.,Yachnin BJ, McEvoy MB, MacCuish RJ, Morley KL, Lau PC, Berghuis AM ACS Chem Biol. 2014 Sep 29. PMID:25265531 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Monooxygenase 3D structures

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