4rpm

Crystal structure of the SAT domain from the non-reducing fungal polyketide synthase CazM with bound hexanoyl

spinqualitylabelsall models 4rpm, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rpm is a 1 chain structure with sequence from Chaetomium globosum CBS 148.51. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4Å Ligands: 6NA, HXC, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q2GWK9_CHAGB Publication Abstract from PubMed Modular collaboration between iterative fungal polyketide synthases (IPKSs) is an important mechanism for generating structural diversity of polyketide natural products. Inter-PKS communication and substrate channeling are controlled in large by the starter unit acyl carrier protein transacylase (SAT) domain found in the accepting IPKS module. Here, we reconstituted the modular biosynthesis of the benzaldehyde core of the chaetoviridin and chaetomugilin azaphilone natural products using the IPKSs CazF and CazM. Our studies revealed a critical role of CazM's SAT domain in selectively transferring a highly reduced triketide product from CazF. In contrast, a more oxidized triketide that is also produced by CazF and required in later stages of biosynthesis of the final product is not recognized by the SAT domain. The structural basis for the acyl unit selectivity was uncovered by the first X-ray structure of a fungal SAT domain, highlighted by a covalent hexanoyl thioester intermediate in the SAT active site. The crystal structure of SAT domain will enable protein engineering efforts aimed at mixing and matching different IPKS modules for the biosynthesis of new compounds. Biochemical and Structural Basis for Controlling Chemical Modularity in Fungal Polyketide Biosynthesis.,Winter JM, Cascio D, Dietrich D, Sato M, Watanabe K, Sawaya MR, Vederas JC, Tang Y J Am Chem Soc. 2015 Aug 12;137(31):9885-93. doi: 10.1021/jacs.5b04520. Epub 2015 , Jul 30. PMID:26172141 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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