4rw3

Structural insights into substrate binding of brown spider venom class II phospholipases D

spinqualitylabelsall models 4rw3, resolution 1.72Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rw3 is a 1 chain structure with sequence from Loxosceles intermedia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.72Å Ligands: DKA, GOL, IPD, MG, OCA, PEG, PLM, SHV, TDA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A1HB2_LOXIN Catalyzes the hydrolysis of sphingomyelin. May also acts on other phosphatidyl esters. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation. Publication Abstract from PubMed Phospholipases D (PLDs) the major dermonecrotic factors from brown spider venoms trigger a range of biological reactions both in vitro and in vivo. Despite their clinical relevance in loxoscelism, structural data is restricted to the apo-form of these enzymes, which has been instrumental in understanding the functional differences between the class I and II spider PLDs. The crystal structures of the native class II PLD from Loxosceles intermedia complexed with myo-inositol 1-phosphate and the inactive mutant H12A complexed with fatty acids indicate the existence of a strong ligand-dependent conformation change of the highly conserved aromatic residues, Tyr 223 and Trp225 indicating their roles in substrate binding. These results provided insights into the structural determinants for substrate recognition and binding by class II PLDs. Structural Insights into Substrate Binding of Brown Spider Venom Class II Phospholipases D.,Coronado MA, Ullah A, da Silva LS, Chaves-Moreira D, Vuitika L, Chaim OM, Veiga SS, Chahine J, Murakami MT, Arni RK Curr Protein Pept Sci. 2015 May 5. PMID:25961401 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phospholipase D 3D structures

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