4tko

Structure of the periplasmic adaptor protein EmrA

spinqualitylabelsall models 4tko, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4tko is a 1 chain structure with sequence from Aquifex aeolicus VF5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.85Å Ligands: IPA, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O67159_AQUAE Publication Abstract from PubMed Periplasmic adaptor proteins are key components of bacterial tripartite efflux pumps. The 2.85A resolution structure of an MFS (major facilitator superfamily) pump adaptor, Aquifex aeolicus EmrA, shows linearly arranged alpha-helical coiled-coil, lipoyl, and beta-barrel domains, but lacks the fourth membrane-proximal domain shown in other pumps to interact with the inner membrane transporter. The adaptor alpha-hairpin, which binds outer membrane TolC, is exceptionally long at 127A, and the beta-barrel contains a conserved disordered loop. The structure extends the view of adaptors as flexible, modular components that mediate diverse pump assembly, and suggests that in MFS tripartite pumps a hexamer of adaptors could provide a periplasmic seal. Structure of the periplasmic adaptor protein from a major facilitator superfamily (MFS) multidrug efflux pump.,Hinchliffe P, Greene NP, Paterson NG, Crow A, Hughes C, Koronakis V FEBS Lett. 2014 Jul 1. pii: S0014-5793(14)00519-5. doi:, 10.1016/j.febslet.2014.06.055. PMID:24996185 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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