4u3q

Crystal Structure of Recombinant TP0435 from Treponema pallidum

spinqualitylabelsall models 4u3q, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4u3q is a 2 chain structure with sequence from Treponema pallidum subsp. pallidum str. Nichols. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TA17_TREPA Publication Abstract from PubMed The sexually transmitted disease syphilis is caused by the bacterial spirochete Treponema pallidum. This microorganism is genetically intractable, accounting for the large number of putative and under-characterized members of the pathogen's proteome. In an effort to ascribe a function(s) to the TP0435 (Tp17) lipoprotein, we engineered a soluble variant of the protein (rTP0435) and determined its crystal structure at a resolution of 2.42 A. The structure is characterized by an eight-stranded beta-barrel protein with a shallow "basin" at one end of the barrel and an alpha-helix stacked on the opposite end. Furthermore, there is a disulfide-linked dimer of the protein in the asymmetric unit of the crystals. Solution hydrodynamic experiments established that purified rTP0435 is monomeric, but specifically forms the disulfide-stabilized dimer observed in the crystal structure. The data herein, when considered with previous work on TP0435, imply plausible roles for the protein in either ligand binding, treponemal membrane architecture, and/or pathogenesis. Insights into the potential function and membrane organization of the TP0435 (Tp17) lipoprotein from Treponema pallidum derived from structural and biophysical analyses.,Brautigam CA, Deka RK, Liu WZ, Norgard MV Protein Sci. 2014 Oct 6. doi: 10.1002/pro.2576. PMID:25287511 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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