4v4h

Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution.

spinqualitylabelsall models 4v4h, resolution 3.46Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v4h is a 20 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 1vs5, 1vs6, 1vs7 and 1vs8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.46Å Ligands: KSG, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS6_ECOLI Binds together with S18 to 16S ribosomal RNA.[HAMAP-Rule:MF_00360] Publication Abstract from PubMed The prokaryotic ribosome is an important target of antibiotic action. We determined the X-ray structure of the aminoglycoside kasugamycin (Ksg) in complex with the Escherichia coli 70S ribosome at 3.5-A resolution. The structure reveals that the drug binds within the messenger RNA channel of the 30S subunit between the universally conserved G926 and A794 nucleotides in 16S ribosomal RNA, which are sites of Ksg resistance. To our surprise, Ksg resistance mutations do not inhibit binding of the drug to the ribosome. The present structural and biochemical results indicate that inhibition by Ksg and Ksg resistance are closely linked to the structure of the mRNA at the junction of the peptidyl-tRNA and exit-tRNA sites (P and E sites). Structural analysis of kasugamycin inhibition of translation.,Schuwirth BS, Day JM, Hau CW, Janssen GR, Dahlberg AE, Cate JH, Vila-Sanjurjo A Nat Struct Mol Biol. 2006 Oct;13(10):879-86. Epub 2006 Sep 24. PMID:16998486 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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