4v83

Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome.

spinqualitylabelsall models 4v83, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v83 is a 20 chain structure with sequence from Thermus thermophilus HB27. This structure supersedes the now removed PDB entries 3pyn, 3pyo, 3pyq and 3pyr. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS6_THET2 Located on the outer edge of the platform on the body of the 30S subunit (By similarity). Publication Abstract from PubMed Internal ribosome entry site (IRES) RNAs are elements of viral or cellular mRNAs that bypass steps of canonical eukaryotic cap-dependent translation initiation. Understanding of the structural basis of IRES mechanisms is limited, partially due to a lack of high-resolution structures of IRES RNAs bound to their cellular targets. Prompted by the universal phylogenetic conservation of the ribosomal P site, we solved the crystal structures of proposed P site binding domains from two intergenic region IRES RNAs bound to bacterial 70S ribosomes. The structures show that these IRES domains nearly perfectly mimic a tRNA*mRNA interaction. However, there are clear differences in the global shape and position of this IRES domain in the intersubunit space compared to those of tRNA, supporting a mechanism for IRES action that invokes hybrid state mimicry to drive a noncanonical mode of translocation. These structures suggest how relatively small structured RNAs can manipulate complex biological machines. Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome.,Zhu J, Korostelev A, Costantino DA, Donohue JP, Noller HF, Kieft JS Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1839-44. Epub 2011 Jan 18. PMID:21245352 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA