4wv3

Crystal structure of the anthranilate CoA ligase AuaEII in complex with anthranoyl-AMP

spinqualitylabelsall models 4wv3, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4wv3 is a 2 chain structure with sequence from Stigmatella aurantiaca. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.599Å Ligands: 3UK Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F3Y661_STIAU Publication Abstract from PubMed The incorporation of nonacetate starter units during type II polyketide biosynthesis helps diversify natural products. Currently, there are few enzymatic strategies for the incorporation of nonacetate starter units in type II polyketide synthase (PKS) pathways. Here we report the crystal structure of AuaEII, the anthranilate:CoA ligase responsible for the generation of anthraniloyl-CoA, which is used as a starter unit by a type II PKS in aurachin biosynthesis. We present structural and protein sequence comparisons to other aryl:CoA ligases. We also compare the AuaEII crystal structure to a model of a CoA ligase homologue, AuaE, which is present in the same gene cluster. AuaE is predicted to have the same fold as AuaEII, but instead of CoA ligation, AuaE catalyzes acyl transfer of anthranilate from anthraniloyl-CoA to the acyl carrier protein (ACP). Together, this work provides insight into the molecular basis for starter unit selection of anthranilate in type II PKS biosynthesis. Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis.,Jackson DR, Tu SS, Nguyen M, Barajas JF, Schaub AJ, Krug D, Pistorius D, Luo R, Muller R, Tsai SC ACS Chem Biol. 2015 Nov 3. PMID:26473393 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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