4xgy

GFP based antibody (fluorobody)

spinqualitylabelsall models 4xgy, resolution 1.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xgy is a 1 chain structure with sequence from Aequorea victoria, Vibrio parahaemolyticus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.494Å Ligands: CRO, PE5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A059PIQ0_AEQVI Publication Abstract from PubMed A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacterium Vibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space group P43212, with unit-cell parameters a = b = 63.35, c = 125.90 A, were obtained by vapour diffusion in hanging drops and the structure was refined to an Rfree of 16.7% at 1.5 A resolution. The structure shows a CDR loop of the antibody on the GFP scaffold. The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.,Chen Y, Huang X, Wang R, Wang S, Shi N Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):913-8. doi:, 10.1107/S2053230X15008845. Epub 2015 Jun 27. PMID:26144238 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Green Fluorescent Protein 3D structures

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