4xxn

Structure of PE-PPE domains of ESX-1 secreted protein EspB, I222

spinqualitylabelsall models 4xxn, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xxn is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.14Å Ligands: CL, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ESPB_MYCTU Publication Abstract from PubMed Mycobacterium tuberculosis secretes multiple virulence factors during infection via the general Sec and Tat pathways, and via specialized ESX secretion systems, also referred to as type VII secretion systems. The ESX-1 secretion system is an important virulence determinant because deletion of ESX-1 leads to attenuation of M. tuberculosis. ESX-1 secreted protein B (EspB) contains putative PE (Pro-Glu) and PPE (Pro-Pro-Glu) domains, and a C-terminal domain, which is processed by MycP1 protease during secretion. We determined the crystal structure of PE-PPE domains of EspB, which represents an all-helical, elongated molecule closely resembling the structure of the PE25-PPE41 heterodimer despite limited sequence similarity. Also, we determined the structure of full-length EspB, which does not have interpretable electron density for the C-terminal domain confirming that it is largely disordered. Comparative analysis of EspB in cell lysate and culture filtrates of M. tuberculosis revealed that mature secreted EspB forms oligomers. Electron microscopy analysis showed that the N-terminal fragment of EspB forms donut-shaped particles. These data provide a rationale for the future investigation of EspB's role in M. tuberculosis pathogenesis. Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis.,Korotkova N, Piton J, Wagner JM, Boy-Rottger S, Japaridze A, Evans TJ, Cole ST, Pojer F, Korotkov KV J Struct Biol. 2015 Jun 4. pii: S1047-8477(15)30007-1. doi:, 10.1016/j.jsb.2015.06.003. PMID:26051906 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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