4ybo

Structure of Citrate Synthase from the Thermoacidophilic Euryarchaeon Thermolasma acidophilum

spinqualitylabelsall models 4ybo, resolution 2.18Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ybo is a 4 chain structure with sequence from Thermoplasma acidophilum DSM 1728. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.179Å Ligands: BCT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CISY_THEAC Publication Abstract from PubMed Citrate synthase (CS) plays a central metabolic role in aerobes and many other organisms. The CS reaction comprises two half-reactions: a Claisen aldol condensation of acetyl-CoA (AcCoA) and oxaloacetate (OAA) that forms citryl-CoA (CitCoA), and CitCoA hydrolysis. Protein conformational changes that `close' the active site play an important role in the assembly of a catalytically competent condensation active site. CS from the thermoacidophile Thermoplasma acidophilum (TpCS) possesses an endogenous Trp fluorophore that can be used to monitor the condensation reaction. The 2.2 A resolution crystal structure of TpCS fused to a C-terminal hexahistidine tag (TpCSH6) reported here is an `open' structure that, when compared with several liganded TpCS structures, helps to define a complete path for active-site closure. One active site in each dimer binds a neighboring His tag, the first nonsubstrate ligand known to occupy both the AcCoA and OAA binding sites. Solution data collectively suggest that this fortuitous interaction is stabilized by the crystalline lattice. As a polar but almost neutral ligand, the active site-tail interaction provides a new starting point for the design of bisubstrate-analog inhibitors of CS. An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase.,Murphy JR, Donini S, Kappock TJ Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1292-9. doi:, 10.1107/S2053230X15015939. Epub 2015 Sep 23. PMID:26457521 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Citrate Synthase 3D structures

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