4yo0

Crystal structure of monoclonal anti-human podoplanin antibody NZ-1 with bound PA peptide

spinqualitylabelsall models 4yo0, resolution 1.56Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4yo0 is a 6 chain structure with sequence from Homo sapiens and Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.56Å Ligands: EDO, PCA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PDPN_HUMAN Publication Abstract from PubMed Placement of a tag sequence is usually limited to either terminal of the target protein, reducing the potential of epitope tags for various labeling applications. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by a high-affinity antibody NZ-1. We determined the crystal structure of the PA tag/NZ-1 complex and found that NZ-1 recognized a central segment of the PA tag peptide in a tight beta-turn configuration, suggesting its compatibility with the insertion into a loop. This possibility was tested and confirmed using multiple integrin subunits and semaphorin. More specifically, the PA tag can be inserted at multiple locations within the alphaIIb subunit of the fibrinogen receptor alphaIIbbeta3 integrin without affecting the structural and functional integrity, while maintaining its high affinity toward NZ-1. The large choice of the sites for "epitope grafting" enabled the placement of the PA tag at a location whose accessibility is modulated during the biological action of the receptor. Thus, we succeeded in converting a general anti-tag antibody into a special reporter/activator anti-beta1 integrin antibody that can be classified as a ligand-induced binding site antibody. Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state.,Fujii Y, Matsunaga Y, Arimori T, Kitago Y, Ogasawara S, Kaneko MK, Kato Y, Takagi J J Cell Sci. 2016 Feb 12. pii: jcs.176685. PMID:26872787 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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