4zbl

Phototoxic fluorescent protein mKillerOrange

spinqualitylabelsall models 4zbl, resolution 1.57Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zbl is a 1 chain structure with sequence from Hydrozoa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.57Å Ligands: 4M9, CIT, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q2TCH5_9CNID Publication Abstract from PubMed Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. Here, we present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and 1.57 A resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the beta-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers. Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.,Pletneva NV, Pletnev VZ, Sarkisyan KS, Gorbachev DA, Egorov ES, Mishin AS, Lukyanov KA, Dauter Z, Pletnev S PLoS One. 2015 Dec 23;10(12):e0145740. doi: 10.1371/journal.pone.0145740., eCollection 2015. PMID:26699366 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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