4zcf

Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I

spinqualitylabelsall models 4zcf, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zcf is a 5 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: AMP, CA, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function T3RE_ECOLX A type III restriction enzyme that recognizes 2 inversely oriented double-stranded sequences 5'-CAGCAG-3' and cleaves DNA 25-27 base pairs downstream of one site. DNA restriction requires both the Res and Mod subunits (PubMed:11178902, PubMed:15464603). DNA topology affects its action; relaxed and negatively supercoiled DNA are digested but positively supercoiled DNA is not a good substrate (PubMed:11178902). Interacts with DNA approximately one half-turn downstream of the recognition site (PubMed:26067164). After binding to one recognition site undergoes random one-dimensional diffusion along DNA until it collides with a stationary enzyme bound to the second DNA site, which is when DNA cleavage occurs (Probable).

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