4zi8
Structure of mouse clustered PcdhgC3 EC1-3
Structural highlights
FunctionQ91XX1_MOUSE Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.[SAAS:SAAS00311819] Publication Abstract from PubMedClustered protocadherin (Pcdh) proteins mediate dendritic self-avoidance in neurons via specific homophilic interactions in their extracellular cadherin (EC) domains. We determined crystal structures of EC1-EC3, containing the homophilic specificity-determining region, of two mouse clustered Pcdh isoforms (PcdhgammaA1 and PcdhgammaC3) to investigate the nature of the homophilic interaction. Within the crystal lattices, we observe antiparallel interfaces consistent with a role in trans cell-cell contact. Antiparallel dimerization is supported by evolutionary correlations. Two interfaces, located primarily on EC2-EC3, involve distinctive clustered Pcdh structure and sequence motifs, lack predicted glycosylation sites, and contain residues highly conserved in orthologs but not paralogs, pointing toward their biological significance as homophilic interaction interfaces. These two interfaces are similar yet distinct, reflecting a possible difference in interaction architecture between clustered Pcdh subfamilies. These structures initiate a molecular understanding of clustered Pcdh assemblies that are required to produce functional neuronal networks. Structure and Sequence Analyses of Clustered Protocadherins Reveal Antiparallel Interactions that Mediate Homophilic Specificity.,Nicoludis JM, Lau SY, Scharfe CP, Marks DS, Weihofen WA, Gaudet R Structure. 2015 Nov 3;23(11):2087-98. doi: 10.1016/j.str.2015.09.005. Epub 2015, Oct 15. PMID:26481813[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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