4zym

Structural implications of homo-pyrimidine base pairs on the parallel-stranded d(GAY) motif.

spinqualitylabelsall models 4zym, resolution 2.53Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zym is a 4 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.53Å Ligands: MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed DNA can adopt many other structures beyond the canonical B-form double helix. There has been a growing interest in using non-canonical base pairs to provide structural diversity for designing DNA architectures for nanotechnology applications. We recently described the crystal structure of d(ACTCGGATGAT) which forms a tetraplex through parallel-stranded homobase pairs and nucleobase intercalation. Here, we examine the structural implications of the homo-pyrimidine base pair within this motif. We have determined crystal structures of two variants that differ from the original structure in the homo-pyrimidine base pairs and number of d(YGA) motifs. Our results show that the intercalation-locked tetraplex motif is predictable and that the substitution of C-C base pairs for T-T base pairs introduces asymmetry to the homoduplex. This hasimplications for utilizing d(YGA) motifs in DNA crystal design and may provide a basis for understanding local structures associated with repeat expansions. Structural implications of homo-pyrimidine base pairs in the parallel-stranded d(YGA) motif.,Tripathi S, Paukstelis PJ Chembiochem. 2015 Dec 2. doi: 10.1002/cbic.201500491. PMID:26629965 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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