5a1a

2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor

Loading... Status: waiting for Mol* initialization Structural highlights 5a1a is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.2Å Ligands: MG, NA, PTQ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BGAL_ECOLI Publication Abstract from PubMed Cryo-electron microscopy (cryo-EM) is rapidly emerging as a powerful tool for protein structure determination at high resolution. Here, we report the structure of a complex between Escherichia coli beta-galactosidase and the cell-permeant inhibitor phenylethyl beta-d-thiogalactopyranoside (PETG), determined by cryo-EM at a resolution of ~2.2 A. Besides the PETG ligand, we identified densities in the map for ~800 water molecules and for magnesium and sodium ions. While it is likely that continued advances in detector technology may further enhance resolution, our findings demonstrate that preparation of specimens of adequate quality and intrinsic protein flexibility, rather than imaging or image processing technologies, now represent the major bottlenecks to achieving resolutions close to 2 A using single particle cryo-EM. 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor.,Bartesaghi A, Merk A, Banerjee S, Matthies D, Wu X, Milne JL, Subramaniam S Science. 2015 May 7. pii: aab1576. PMID:25953817 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Galactosidase 3D structures

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