5b4s

Crystal Structure of GH80 chitosanase from Mitsuaria chitosanitabida

spinqualitylabelsall models 5b4s, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5b4s is a 2 chain structure with sequence from Mitsuaria chitosanitabida. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O82856_9BURK Publication Abstract from PubMed Chitosanases belong to glycoside hydrolase families 5, 7, 8, 46, 75 and 80 and hydrolyse glucosamine polymers produced by partial or full deacetylation of chitin. Herein, we determined the crystal structure of chitosanase from the beta-proteobacterium, Mitsuaria chitosanitabida, (McChoA) at 1.75 A resolution; the first structure of a family 80 chitosanase. McChoA is a 34 kDa extracellular protein of 301 amino acids that fold into two (upper and lower) globular domains with an active site cleft between them. Key substrate-binding features are conserved with family 24 lysozymes and family 46 chitosanases. The distance between catalytic residues E41 and E61 (10.8 A) indicates an inverting type mechanism. Uniquely, three disulphide bridges and the C terminus might contribute to enzyme activity. Crystal structure of a family 80 chitosanase from Mitsuaria chitosanitabida.,Yorinaga Y, Kumasaka T, Yamamoto M, Hamada K, Kawamukai M FEBS Lett. 2017 Jan 13. doi: 10.1002/1873-3468.12557. PMID:28084023 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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