5bjr

Crystal structure of the N-terminal RRM domain from MEC-8

spinqualitylabelsall models 5bjr, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5bjr is a 2 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: NH4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G5ECJ4_CAEEL Publication Abstract from PubMed Precise regulation of mRNA processing, translation, localization and stability relies on specific interactions with RNA-binding proteins whose biological function and target preference are dictated by their preferred RNA motifs. The RBPMS family of RNA-binding proteins is defined by a conserved RNA recognition motif (RRM) domain found in metazoan RBPMS/Hermes and RBPMS2, Drosophila couch potato, and MEC-8 from C. elegans. In order to determine the parameters of RNA sequence recognition by the RBPMS family, we have first used the N-terminal domain from MEC-8 in binding assays and have demonstrated a preference for two GCAC motifs optimally separated by greater than six nucleotides. We have also determined the crystal structure of the dimeric N-terminal RRM domain from MEC-8 in the unbound form, and in complex with an oligonucleotide harbouring two copies of the optimal GCAC motif. The atomic details reveal the molecular network that provides specificity to all four bases in the motif, including multiple hydrogen bonds to the initial guanine. Further studies with human RBPMS, as well as Drosophila couch potato, confirm a general preference for this double GCAC motif by other members of the protein family and the presence of this motif in known targets. Conserved binding of GCAC motifs by MEC-8, couch potato and the RBPMS protein family.,Soufari H, Mackereth CD RNA. 2016 Dec 21. pii: rna.059733.116. doi: 10.1261/rna.059733.116. PMID:28003515 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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