5dm9
XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedA liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 microm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 A from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption. Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography.,Mafune F, Miyajima K, Tono K, Takeda Y, Kohno JY, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S, Yabashi M Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):520-3. doi:, 10.1107/S2059798316001480. Epub 2016 Mar 24. PMID:27050131[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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