5drv

Crystal structure of the G3BP2 NTF2-like domain in complex with a peptide

spinqualitylabelsall models 5drv, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5drv is a 2 chain structure with sequence from Homo sapiens and Semliki Forest virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.75Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G3BP2_HUMAN Probable scaffold protein that may be involved in mRNA transport. Publication Abstract from PubMed The crystal structure of the NTF2-like domain of the human Ras GTPase SH3 Binding Protein (G3BP), isoform 2, was determined at a resolution of 2.75 A in complex with a peptide containing a FGDF sequence motif. The overall structure of the protein is highly similar to the homodimeric N-terminal domains of the G3BP1 and Rasputin proteins. Recently, a subset of G3BP interacting proteins was recognized to share a common sequence motif, FGDF. The most studied binding partners, USP10 and viral nsP3, interfere with essential G3BP functions related to assembly of cellular stress granules. Reported molecular modeling suggested that FGDF-motif containing peptides bind in an extended conformation into a hydrophobic groove on the surface of the G3BP NTF2-like domain in a manner similar to the known binding of FxFG nucleoporin repeats. The results in this paper provide evidence for a different binding mode. The FGDF peptide binds and changes conformation of the protruding N-terminal residues by providing hydrophobic interactions to a symmetry related molecule that facilitated crystallization of the G3BP2 isoform. Crystal structure of the G3BP2 NTF2-like domain in complex with a canonical FGDF motif peptide.,Kristensen O Biochem Biophys Res Commun. 2015 Sep 26. pii: S0006-291X(15)30640-9. doi:, 10.1016/j.bbrc.2015.09.123. PMID:26410532 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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