5f0o

Cohesin subunit Pds5 in complex with Scc1

spinqualitylabelsall models 5f0o, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5f0o is a 2 chain structure with sequence from Lachancea thermotolerans and Lachancea thermotolerans CBS 6340. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C5DGP8_LACTC Publication Abstract from PubMed Sister chromatid cohesion is mediated by cohesin, whose Smc1, Smc3, and kleisin (Scc1) subunits form a ring structure that entraps sister DNAs. The ring is opened either by separase, which cleaves Scc1 during anaphase, or by a releasing activity involving Wapl, Scc3, and Pds5, which bind to Scc1 and open its interface with Smc3. We present crystal structures of Pds5 from the yeast L. thermotolerans in the presence and absence of the conserved Scc1 region that interacts with Pds5. Scc1 binds along the spine of the Pds5 HEAT repeat fold and is wedged between the spine and C-terminal hook of Pds5. We have isolated mutants that confirm the observed binding mode of Scc1 and verified their effect on cohesin by immunoprecipitation and calibrated ChIP-seq. The Pds5 structure also reveals architectural similarities to Scc3, the other large HEAT repeat protein of cohesin and, most likely, Scc2. Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1.,Lee BG, Roig MB, Jansma M, Petela N, Metson J, Nasmyth K, Lowe J Cell Rep. 2016 Mar 8;14(9):2108-15. doi: 10.1016/j.celrep.2016.02.020. Epub 2016 , Feb 25. PMID:26923598 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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