5fpz

The structure of KdgF from Yersinia enterocolitica with malonate bound in the active site.

spinqualitylabelsall models 5fpz, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5fpz is a 1 chain structure with sequence from Yersinia enterocolitica subsp. enterocolitica 8081. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Ligands: MLA, NI Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A1JMF7_YERE8 Publication Abstract from PubMed Uronates are charged sugars that form the basis of two abundant sources of biomass-pectin and alginate-found in the cell walls of terrestrial plants and marine algae, respectively. These polysaccharides represent an important source of carbon to those organisms with the machinery to degrade them. The microbial pathways of pectin and alginate metabolism are well studied and essentially parallel; in both cases, unsaturated monouronates are produced and processed into the key metabolite 2-keto-3-deoxygluconate (KDG). The enzymes required to catalyze each step have been identified within pectinolytic and alginolytic microbes; yet the function of a small ORF, kdgF, which cooccurs with the genes for these enzymes, is unknown. Here we show that KdgF catalyzes the conversion of pectin- and alginate-derived 4,5-unsaturated monouronates to linear ketonized forms, a step in uronate metabolism that was previously thought to occur spontaneously. Using enzyme assays, NMR, mutagenesis, and deletion of kdgF, we show that KdgF proteins from both pectinolytic and alginolytic bacteria catalyze the ketonization of unsaturated monouronates and contribute to efficient production of KDG. We also report the X-ray crystal structures of two KdgF proteins and propose a mechanism for catalysis. The discovery of the function of KdgF fills a 50-y-old gap in the knowledge of uronate metabolism. Our findings have implications not only for the understanding of an important metabolic pathway, but also the role of pectinolysis in plant-pathogen virulence and the growing interest in the use of pectin and alginate as feedstocks for biofuel production. KdgF, the missing link in the microbial metabolism of uronate sugars from pectin and alginate.,Hobbs JK, Lee SM, Robb M, Hof F, Barr C, Abe KT, Hehemann JH, McLean R, Abbott DW, Boraston AB Proc Natl Acad Sci U S A. 2016 May 16. pii: 201524214. PMID:27185956 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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