5g5r

CBS domain tandem of site-2 protease from Archaeoglobus fulgidus in complex with llama Nanobody - apo form

spinqualitylabelsall models 5g5r, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5g5r is a 2 chain structure with sequence from Archaeoglobus fulgidus and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O29915_ARCFU Publication Abstract from PubMed Regulated intramembrane proteolysis by members of the site-2 protease family (S2P) is an essential signal transduction mechanism conserved from bacteria to humans. There is some evidence that extra-membranous domains, like PDZ and CBS domains, regulate the proteolytic activity of S2Ps and that some members act as dimers. Here we report the crystal structure of the regulatory CBS domain pair of S2P from Archaeoglobus fulgidus, AfS2P, in the apo and nucleotide-bound form in complex with a specific Nanobody from llama. Cross-linking and SEC-MALS analyses show for the first time the dimeric architecture of AfS2P both in the membrane and in detergent micelles. The CBS domain pair dimer (CBS module) displays an unusual head-to-tail configuration and nucleotide binding triggers no major conformational changes in the magnesium-free state. In solution, MgATP drives monomerization of the CBS module. We propose a model of the so far unknown architecture of the transmembrane domain dimer and for a regulatory mechanism of AfS2P that involves the interaction of positively charged arginine residues located at the cytoplasmic face of the transmembrane domain with the negatively charged phosphate groups of ATP moieties bound to the CBS domain pairs. Binding of MgATP could promote opening of the CBS module to allow lateral access of the globular cytoplasmic part of the substrate. Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease.,Schacherl M, Gompert M, Pardon E, Lamkemeyer T, Steyaert J, Baumann U Biochim Biophys Acta. 2017 May 11. pii: S0005-2736(17)30159-1. doi:, 10.1016/j.bbamem.2017.05.006. PMID:28502790 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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