5gw9

Crystal structure of C163, a backbone circularized G-CSF

spinqualitylabelsall models 5gw9, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5gw9 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.65Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CSF3_HUMAN Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Publication Abstract from PubMed Backbone circularization is a powerful approach for enhancing the structural stability of polypeptides. Herein, we present the crystal structure of the circularized variant of the granulocyte colony-stimulating factor (G-CSF) in which the terminal helical region was circularized using a short, two-amino acid connector. The structure revealed that the N- and C-termini were indeed connected by a peptide bond. The local structure of the C-terminal region transited from an alpha helix to 310 helix with a bend close to the N-terminal region, indicating that the structural change offset the insufficient length of the connector. This is the first-ever report of a crystal structure of the backbone of a circularized protein. It will facilitate the development of backbone circularization methodology. Structural insights into the backbone-circularized granulocyte colony-stimulating factor containing a short connector.,Miyafusa T, Shibuya R, Honda S Biochem Biophys Res Commun. 2018 Apr 16. pii: S0006-291X(18)30808-8. doi:, 10.1016/j.bbrc.2018.04.045. PMID:29634929 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Colony-stimulating factor 3D structures

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