5h3v

Crystal structure of a Type IV Secretion System Component CagX in Helicobacter pylori

spinqualitylabelsall models 5h3v, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5h3v is a 2 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4Å Ligands: IPA, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O25263_HELPY Publication Abstract from PubMed Helicobacter pylori, a Gram-negative bacterial pathogen prevalent in the human population, is the causative agent of severe gastric diseases. An H. pylori type IV secretion (T4S) system encoded by the cytotoxin-associated gene pathogenicity island (cagPAI) is responsible for communication with host cells. As a component of the cagPAI T4S system core complex, CagX plays an important role in virulence-protein translocation into the host cells. In this work, the crystal structure of the C-terminal domain of CagX (CagXct), which is a homologue of the VirB9 protein from the VirB/D4 T4S system, is presented. CagXct is only the second three-dimensional structure to be elucidated of a VirB9-like protein. Another homologue, TraO, which is encoded on the Escherichia coli conjugative plasmid pKM101, shares only 19% sequence identity with CagXct; however, there is a remarkable similarity in tertiary structure between these two beta-sandwich protein domains. Most of the residues that are conserved between CagXct and TraO are located within the protein core and appear to be responsible for the preservation of this domain fold. The studies presented here will contribute to our understanding of different bacterial T4S systems. Crystal structure of the type IV secretion system component CagX from Helicobacter pylori.,Zhang J, Fan F, Zhao Y, Sun L, Liu Y, Keegan RM, Isupov MN, Wu Y Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):167-173. doi:, 10.1107/S2053230X17001376. Epub 2017 Feb 28. PMID:28291753 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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