5hd2

The crystal structure of SeMet-Cry51Aa2-L11M

spinqualitylabelsall models 5hd2, resolution 2.28Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hd2 is a 1 chain structure with sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.276Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E9KBU4_BACTU Publication Abstract from PubMed Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts. A transgenic approach for controlling Lygus in cotton.,Gowda A, Rydel TJ, Wollacott AM, Brown RS, Akbar W, Clark TL, Flasinski S, Nageotte JR, Read AC, Shi X, Werner BJ, Pleau MJ, Baum JA Nat Commun. 2016 Jul 18;7:12213. doi: 10.1038/ncomms12213. PMID:27426014 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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