5hiw

Sorangium cellulosum So Ce56 cytochrome P450 260B1

spinqualitylabelsall models 5hiw, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hiw is a 1 chain structure with sequence from Sorangium cellulosum So ce56. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: HEM, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A9FFA1_SORC5 Publication Abstract from PubMed Myxobacterial CYP260B1 from Sorangium cellulosum was heterologously expressed in Escherichia coli and purified. The in vitro conversion of a small focused substrate library comprised of Delta4 C21-steroids and steroidal drugs using surrogate bovine redox partners shows that CYP260B1 is a novel steroid hydroxylase. CYP260B1 performs the regio- and stereoselective hydroxylation of the glucocorticoid cortodoxone (RSS) to produce 6beta-OH-RSS. The substrate-free crystal structure of CYP260B1 (PDB 5HIW) was resolved. Docking of the tested ligands into the crystal structure suggested that the C17 hydroxy moiety and the presence of either a keto or a hydroxy group at C11 determine the selectivity of hydroxylation. Structure-function analysis for the hydroxylation of Delta4 C21-steroids by the myxobacterial CYP260B1.,Salamanca-Pinzon SG, Khatri Y, Carius Y, Keller L, Muller R, Lancaster CR, Bernhardt R FEBS Lett. 2016 Jun;590(12):1838-51. doi: 10.1002/1873-3468.12217. Epub 2016 Jun , 3. PMID:27177597 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome P450 3D structures

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