5i3c

Crystal structure of E.coli purine nucleoside phosphorylase with acycloguanosine

spinqualitylabelsall models 5i3c, resolution 2.32Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5i3c is a 3 chain structure with sequence from Escherichia coli O139:H28 str. E24377A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.32Å Ligands: AC2, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DEOD_ECO24 Publication Abstract from PubMed Escherichia coli purine nucleoside phosphorylase (PNP), which catalyzes the reversible phosphorolysis of purine ribonucleosides, belongs to the family I hexameric PNPs. Owing to their key role in the purine salvage pathway, PNPs are attractive targets for drug design against some pathogens. Acyclovir (ACV) is an acyclic derivative of the PNP substrate guanosine and is used as an antiviral drug for the treatment of some human viral infections. The crystalline complex of E. coli PNP with acyclovir was prepared by co-crystallization in microgravity using counter-diffusion through a gel layer in a capillary. The structure of the E. coli PNP-ACV complex was solved at 2.32 A resolution using the molecular-replacement method. The ACV molecule is observed in two conformations and sulfate ions were located in both the nucleoside-binding and phosphate-binding pockets of the enzyme. A comparison with the complexes of other hexameric and trimeric PNPs with ACV shows the similarity in acyclovir binding by these enzymes. Crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with acyclovir.,Timofeev VI, Zhukhlistova NE, Abramchik YA, Muravieva TI, Esipov RS, Kuranova IP Acta Crystallogr F Struct Biol Commun. 2018 Jul 1;74(Pt 7):402-409. doi:, 10.1107/S2053230X18008087. Epub 2018 Jun 26. PMID:29969103 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Purine nucleoside phosphorylase 3D structures

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