5i8i

Crystal Structure of the K. lactis Urea Amidolyase

spinqualitylabelsall models 5i8i, resolution 6.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5i8i is a 4 chain structure with sequence from Kluyveromyces lactis NRRL Y-1140. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 6.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6CP22_KLULA Publication Abstract from PubMed Urea is the degradation product of a wide range of nitrogen containing bio-molecules. Urea amidolyase (UA) catalyzes the conversion of urea to ammonium, the essential first step in utilizing urea as a nitrogen source. It is widely distributed in fungi, bacteria and other microorganisms, and plays an important role in nitrogen recycling in the biosphere. UA is composed of urea carboxylase (UC) and allophanate hydrolase (AH) domains, which catalyze sequential reactions. In some organisms UC and AH are encoded by separated genes. We present here structure of the Kluyveromyces lactis UA (KlUA). The structure revealed that KlUA forms a compact homo-dimer with a molecular weight of 400 kDa. Structure inspired biochemical experiments revealed the mechanism of its reaction intermediate translocation, and that the KlUA holo-enzyme formation is essential for its optimal activity. Interestingly, previous studies and ours suggest that UC and AH encoded by separated genes probably do not form a KlUA-like complex, consequently they might not catalyze the urea to ammonium conversion as efficiently. Structure and Function of Urea Amidolyase.,Zhao J, Zhu L, Fan C, Wu Y, Xiang S Biosci Rep. 2017 Dec 20. pii: BSR20171617. doi: 10.1042/BSR20171617. PMID:29263142 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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