5k5d

Structure of the C2221 form of Pnob8-like ParB-N domain

spinqualitylabelsall models 5k5d, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5k5d is a 3 chain structure with sequence from Saccharolobus solfataricus 98/2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.45Å Ligands: CIT, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D0KSP7_SACS9 Publication Abstract from PubMed Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life. Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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