5kei

Mycobacterium smegmatis MbtA apo structure

spinqualitylabelsall models 5kei, resolution 2.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5kei is a 1 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.325Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0R0V0_MYCS2 Publication Abstract from PubMed Iron is an essential element for life, but its soluble form is scarce in the environment and is rarer in the human body. Mycobacterium tuberculosis (Mtb) produces two aryl-capped siderophores, mycobactin (MBT) and carboxymycobactin (cMBT), to chelate intracellular iron. The adenylating enzyme MbtA catalyzes the first step of mycobactin biosynthesis in two half-reactions: activation of the salicylic acid as an acyl-adenylate and ligation onto the acyl carrier protein (ACP) domain of MbtB to form covalently salicylated MbtB-ACP. We report the first apo-MbtA structure from Mycobacterium smegmatis at 2.3 A. We demonstrate here that MbtA activity can be reversibly, post-translationally regulated by acetylation. Indeed the mycobacterial protein acetyltransferase (Pat), Rv0998, specifically acetylates MbtA on lysine 546, in a cAMP-dependent manner, leading to enzyme inhibition. MbtA acetylation can be reversed by the NAD+-dependant deacetyltransferase, Rv1151c (DAc). Deletion of Pat and DAc genes in Mtb revealed distinct phenotypes for strains lacking one or the other genes at low pH and limiting iron conditions. This study establishes a direct connection between the reversible acetylation system Pat/DAc and the ability of Mtb to adapt in limited iron conditions, which is critical for mycobacterial infection. Post-Translational Acetylation of MbtA Modulates Mycobacterial Siderophore Biosynthesis.,Vergnolle O, Xu H, Tufariello JM, Favrot L, Malek AA, Jacobs WR Jr, Blanchard JS J Biol Chem. 2016 Aug 26. pii: jbc.M116.744532. PMID:27566542 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA