5l2a

Structure of CNTnw N149S,F366A in an outward-facing state

spinqualitylabelsall models 5l2a, resolution 3.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5l2a is a 3 chain structure with sequence from Neisseria wadsworthii 9715. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.45Å Ligands: 6ZL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G4CRQ5_9NEIS Publication Abstract from PubMed Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion. Visualizing multistep elevator-like transitions of a nucleoside transporter.,Hirschi M, Johnson ZL, Lee SY Nature. 2017 Apr 17. doi: 10.1038/nature22057. PMID:28424521 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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