5l2r

Crystal structure of fumarate hydratase from Leishmania major

spinqualitylabelsall models 5l2r, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5l2r is a 2 chain structure with sequence from Leishmania major. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.054Å Ligands: GOL, LMR, MLA, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FUM2_LEIMA Publication Abstract from PubMed Fumarate hydratases (FHs) are essential metabolic enzymes grouped into two classes. Here, we present the crystal structure of a class I FH, the cytosolic FH from Leishmania major, which reveals a previously undiscovered protein fold that coordinates a catalytically essential [4Fe-4S] cluster. Our 2.05 A resolution data further reveal a dimeric architecture for this FH that resembles a heart, with each lobe comprised of two domains that are arranged around the active site. Besides the active site, where the substrate S-malate is bound bidentate to the unique iron of the [4Fe-4S] cluster, other binding pockets are found near the dimeric enzyme interface, some of which are occupied by malonate, shown here to be a weak inhibitor of this enzyme. Taken together, these data provide a framework both for investigations of the class I FH catalytic mechanism and for drug design aimed at fighting neglected tropical diseases. Crystal structure of an Fe-S cluster-containing fumarate hydratase enzyme from Leishmania major reveals a unique protein fold.,Feliciano PR, Drennan CL, Nonato MC Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9804-9. doi:, 10.1073/pnas.1605031113. Epub 2016 Aug 15. PMID:27528683 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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