5lcb

In situ atomic-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum obtained combining solid-state NMR spectroscopy, cryo electron microscopy and polarization spectroscopy

Loading... Status: waiting for Mol* initialization Structural highlights 5lcb is a 14 chain structure with sequence from Chlorobaculum tepidum TLS. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy , Hybrid , Solid-state NMR, Resolution 26.5Å, 1 model Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CSMA_CHLTE Component of the photosynthetic apparatus. The light harvesting B740 complex binds bacteriochlorophyll c. Publication Abstract from PubMed Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix. In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum.,Nielsen JT, Kulminskaya NV, Bjerring M, Linnanto JM, Ratsep M, Pedersen MO, Lambrev PH, Dorogi M, Garab G, Thomsen K, Jegerschold C, Frigaard NU, Lindahl M, Nielsen NC Nat Commun. 2016 Aug 18;7:12454. doi: 10.1038/ncomms12454. PMID:27534696 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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