5lin
Lysozyme, collected at rotation 1 degree per second
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedThe development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 x 75 microm, frame rates of up to 3000 Hz and a dead time as low as 3.8 micros. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine varphi-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed. EIGER detector: application in macromolecular crystallography.,Casanas A, Warshamanage R, Finke AD, Panepucci E, Olieric V, Noll A, Tampe R, Brandstetter S, Forster A, Mueller M, Schulze-Briese C, Bunk O, Wang M Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1036-48. doi:, 10.1107/S2059798316012304. Epub 2016 Aug 31. PMID:27599736[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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