5m1j

Nonstop ribosomal complex bound with Dom34 and Hbs1

Loading... Status: waiting for Mol* initialization Structural highlights 5m1j is a 10 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.3Å Ligands: 1MA, 2MG, 5CR, 5MC, 5MU, 7MG, GNP, H2U, M2G, MG, OMC, OMG, PSU, YYG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS24A_YEAST Publication Abstract from PubMed The surveillance of mRNA translation is imperative for homeostasis. Monitoring the integrity of the message is essential, as the translation of aberrant mRNAs leads to stalling of the translational machinery. During ribosomal rescue, arrested ribosomes are specifically recognized by the conserved eukaryotic proteins Dom34 and Hbs1, to initiate their recycling. Here we solve the structure of Dom34 and Hbs1 bound to a yeast ribosome programmed with a nonstop mRNA at 3.3 A resolution using cryo-electron microscopy. The structure shows that Domain N of Dom34 is inserted into the upstream mRNA-binding groove via direct stacking interactions with conserved nucleotides of 18S rRNA. It senses the absence of mRNA at the A-site and part of the mRNA entry channel by direct competition. Thus, our analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34.Hbs1.GTP complex during Dom34-mediated mRNA surveillance pathways. Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.,Hilal T, Yamamoto H, Loerke J, Burger J, Mielke T, Spahn CM Nat Commun. 2016 Dec 20;7:13521. doi: 10.1038/ncomms13521. PMID:27995908 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 3D sructureseceptor for activated protein kinase C 1

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