5m3z

Crystal structure of Citrobacter freundii methionine gamma-lyase with C115H replacement in the complex with L-norleucine

spinqualitylabelsall models 5m3z, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5m3z is a 1 chain structure with sequence from Citrobacter freundii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.45Å Ligands: CSO, NLE, PGE, PLP, PY6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0A5P8W7_CITFR Publication Abstract from PubMed The mutant form of Citrobacter freundii methionine gamma-lyase with the replacement of active site Cys115 for His has been found to be inactive in the gamma-elimination reaction of methionine while fully active in the gamma-elimination reaction of O-acetyl-l-homoserine and in the beta-elimination reaction of S-alk(en)yl-substituted cysteines. In this work, the crystal structure of the mutant enzyme complexed with competitive inhibitor, l-norleucine was determined at 1.45A resolution. At the enzyme active site the inhibitor proved to be bound both noncovalently and covalently, which corresponds to the two intermediates of the gamma- and beta-elimination reactions, Michaelis complex and the external aldimine. Analysis of the structure allowed us to suggest the possible reason for the inability of the mutant enzyme to catalyze the physiological reaction. Crystal structure of mutant form Cys115His of Citrobacter freundii methionine gamma-lyase complexed with l-norleucine.,Revtovich SV, Morozova EA, Kulikova VV, Anufrieva NV, Osipova TI, Koval VS, Nikulin AD, Demidkina TV Biochim Biophys Acta. 2017 Sep;1865(9):1123-1128. doi:, 10.1016/j.bbapap.2017.06.001. Epub 2017 Jun 6. PMID:28602917 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Methionine gamma-lyase 3D structures

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