5n9y

The full-length structure of ZntB

Loading... Status: waiting for Mol* initialization Structural highlights 5n9y is a 5 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.2Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ZNTB_ECOLI Mediates efflux of zinc ions.[HAMAP-Rule:MF_01565] Publication Abstract from PubMed Zinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic, therefore dedicated import, export and storage proteins for tight regulation of the zinc concentration have evolved. In Enterobacteriaceae, several membrane transporters are involved in zinc homeostasis and linked to virulence. ZntB has been proposed to play a role in the export of zinc, but the transport mechanism of ZntB is poorly understood and based only on experimental characterization of its distant homologue CorA magnesium channel. Here, we report the cryo-electron microscopy structure of full-length ZntB from Escherichia coli together with the results of isothermal titration calorimetry, and radio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes. Our results show that ZntB mediates Zn2+ uptake, stimulated by a pH gradient across the membrane, using a transport mechanism that does not resemble the one proposed for homologous CorA channels. The structural basis of proton driven zinc transport by ZntB.,Gati C, Stetsenko A, Slotboom DJ, Scheres SHW, Guskov A Nat Commun. 2017 Nov 3;8(1):1313. doi: 10.1038/s41467-017-01483-7. PMID:29101379 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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