5nl1

Shigella IpaA-VBS3/TBS in complex with the Talin VBS1 domain 488-512

spinqualitylabelsall models 5nl1, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nl1 is a 12 chain structure with sequence from Mus musculus and Shigella flexneri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: CL, EDO, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TLN1_MOUSE Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Publication Abstract from PubMed The Shigella type III effector IpaA contains three binding sites for the focal adhesion protein vinculin (VBSs), which are involved in bacterial invasion of host cells. Here, we report that IpaA VBS3 unexpectedly binds to talin. The 2.5 A resolution crystal structure of IpaA VBS3 in complex with the talin H1-H4 helices shows a tightly folded alpha-helical bundle, which is in contrast to the bundle unraveling upon vinculin interaction. High-affinity binding to talin H1-H4 requires a core of hydrophobic residues and electrostatic interactions conserved in talin VBS H46. Remarkably, IpaA VBS3 localizes to filopodial distal adhesions enriched in talin, but not vinculin. In addition, IpaA VBS3 binding to talin was required for filopodial adhesions and efficient capture of Shigella. These results point to the functional diversity of VBSs and support a specific role for talin binding by a subset of VBSs in the formation of filopodial adhesions. Shigella IpaA Binding to Talin Stimulates Filopodial Capture and Cell Adhesion.,Valencia-Gallardo C, Bou-Nader C, Aguilar-Salvador DI, Carayol N, Quenech'Du N, Pecqueur L, Park H, Fontecave M, Izard T, Tran Van Nhieu G Cell Rep. 2019 Jan 22;26(4):921-932.e6. doi: 10.1016/j.celrep.2018.12.091. PMID:30673614 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Talin 3D structures

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