5nva

Substrate-bound outward-open state of a Na+-coupled sialic acid symporter reveals a novel Na+-site

spinqualitylabelsall models 5nva, resolution 2.26Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nva is a 1 chain structure with sequence from Proteus mirabilis HI4320. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.26Å Ligands: NA, SLB Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B4EZY7_PROMH Publication Abstract from PubMed Many pathogenic bacteria utilise sialic acids as an energy source or use them as an external coating to evade immune detection. As such, bacteria that colonise sialylated environments deploy specific transporters to mediate import of scavenged sialic acids. Here, we report a substrate-bound 1.95 A resolution structure and subsequent characterisation of SiaT, a sialic acid transporter from Proteus mirabilis. SiaT is a secondary active transporter of the sodium solute symporter (SSS) family, which use Na(+) gradients to drive the uptake of extracellular substrates. SiaT adopts the LeuT-fold and is in an outward-open conformation in complex with the sialic acid N-acetylneuraminic acid and two Na(+) ions. One Na(+) binds to the conserved Na2 site, while the second Na(+) binds to a new position, termed Na3, which is conserved in many SSS family members. Functional and molecular dynamics studies validate the substrate-binding site and demonstrate that both Na(+) sites regulate N-acetylneuraminic acid transport. Substrate-bound outward-open structure of a Na(+)-coupled sialic acid symporter reveals a new Na(+) site.,Wahlgren WY, Dunevall E, North RA, Paz A, Scalise M, Bisignano P, Bengtsson-Palme J, Goyal P, Claesson E, Caing-Carlsson R, Andersson R, Beis K, Nilsson UJ, Farewell A, Pochini L, Indiveri C, Grabe M, Dobson RCJ, Abramson J, Ramaswamy S, Friemann R Nat Commun. 2018 May 1;9(1):1753. doi: 10.1038/s41467-018-04045-7. PMID:29717135 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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