5nxq

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to a stapled Sld5 CIP

spinqualitylabelsall models 5nxq, resolution 2.41Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nxq is a 5 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.413Å Ligands: 9FZ, 9G2, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CTF4_YEAST Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo. Publication Abstract from PubMed Exploitation of synthetic lethality by small-molecule targeting of pathways that maintain genomic stability is an attractive chemotherapeutic approach. The Ctf4/AND-1 protein hub that links DNA replication, repair and chromosome segregation, represents a novel target for the synthetic lethality approach. Here we report the design, optimization, and validation of double-click stapled peptides encoding the Ctf4-interacting peptide (CIP) of the replicative helicase subunit Sld5. Screening stapling positions in the Sld5 CIP, we identified an unorthodox i,i+6 stapled peptide with improved, sub-micromolar binding to Ctf4. The mode of interaction with Ctf4 was confirmed by a crystal structure of the stapled Sld5 peptide bound to Ctf4. The stapled Sld5 peptide was able to displace the Ctf4-partner DNA polymerase alpha from the replisome in yeast extracts. Our study provides proof-of-principle evidence for the development of small-molecule inhibitors of the human-CTF4 orthologue AND-1. Targeting the genome stability hub Ctf4 by stapled-peptide design.,Wu Y, Villa F, Maman J, Dobnikar L, Lau YH, Simon AC, Labib K, Spring DR, Pellegrini L Angew Chem Int Ed Engl. 2017 Aug 17. doi: 10.1002/anie.201705611. PMID:28815832 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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