5nz8

Clostridium thermocellum cellodextrin phosphorylase with cellotetraose and phosphate bound

spinqualitylabelsall models 5nz8, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5nz8 is a 2 chain structure with sequence from Acetivibrio thermocellus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Ligands: BGC, PO4, PRD_900011 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q93HT8_ACETH Publication Abstract from PubMed The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4.1.49) produces cellodextrin oligomers from short beta-1-->4-glucans and alpha-D-glucose 1-phosphate. Compared to cellobiose phosphorylase (CBP), which produces cellobiose from glucose and alpha-D-glucose 1-phosphate, CDP is biochemically less well characterised. Herein, we investigate the donor and acceptor substrate specificity of recombinant CDP from Ruminiclostridium thermocellum and we isolate and characterise a glucosamine addition product to the cellobiose acceptor with the non-natural donor alpha-D-glucosamine 1-phosphate. In addition, we report the first X-ray crystal structure of CDP, along with comparison to the available structures from CBPs and other closely related enzymes, which contributes to understanding of the key structural features necessary to discriminate between monosaccharide (CBP) and oligosaccharide (CDP) acceptor substrates. Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis.,O'Neill EC, Pergolizzi G, Stevenson CEM, Lawson DM, Nepogodiev SA, Field RA Carbohydr Res. 2017 Jul 21. pii: S0008-6215(17)30359-2. doi:, 10.1016/j.carres.2017.07.005. PMID:28760417 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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