5o60

Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis

Loading... Status: waiting for Mol* initialization Structural highlights 5o60 is a 10 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.203Å Ligands: MG, PHE, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0QTP4_MYCS2 Publication Abstract from PubMed The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-A cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.,Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N Cell Rep. 2017 Jul 5;20(1):149-160. doi: 10.1016/j.celrep.2017.06.029. PMID:28683309 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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