5oe0

CRYSTAL STRUCTURE OF THE BETA-LACTAMASE OXA-181

spinqualitylabelsall models 5oe0, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5oe0 is a 2 chain structure with sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.0500083Å Ligands: CL, KCX, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G5CKK8_KLEPN Publication Abstract from PubMed The first crystal structures of the class D beta-lactamases OXA-181 and OXA-245 were determined to 2.05 and 2.20 A resolution, respectively; in addition, the structure of a new crystal form of OXA-163 was resolved to 2.07 A resolution. All of these enzymes are OXA-48-like and have been isolated from different clinical Klebsiella pneumoniae strains and also from other human pathogens such as Pseudomonas aeruginosa and Escherichia coli. Here, enzyme kinetics and thermostability studies are presented, and the new crystal structures are used to explain the observed variations. OXA-245 had the highest melting point (Tm = 55.8 degrees C), as determined by differential scanning calorimetry, compared with OXA-163 (Tm = 49.4 degrees C) and OXA-181 (Tm = 52.6 degrees C). The differences could be explained by the loss of two salt bridges in OXA-163, and an overall decrease in the polarity of the surface of OXA-181 compared with OXA-245. Structure, activity and thermostability investigations of OXA-163, OXA-181 and OXA-245 using biochemical analysis, crystal structures and differential scanning calorimetry analysis.,Lund BA, Thomassen AM, Carlsen TJO, Leiros HKS Acta Crystallogr F Struct Biol Commun. 2017 Oct 1;73(Pt 10):579-587. doi:, 10.1107/S2053230X17013838. Epub 2017 Oct 2. PMID:28994407 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-lactamase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA